webpage api button
Multilineage potential of cells from the artery wall - Spinal DISC Center | Kris Radcliff, MD | New Jersey
Dr. Kris Radcliff specializes in simplifying the management of complex spine conditions and traumatic spine injuries, focusing exclusively on spine surgery, with particular expertise in the area of artificial disc replacement. Dr. Radcliff is highly experienced, having performed more than 10,000 spine surgeries. He combines conservative decision-making judgment with state-of-the-art and minimally invasive surgical techniques, endoscopic spine surgery, and artificial disc replacement.
Dr. Kris Radcliff, Kris Radcliff MD, Kris Radcliff, spinal disc center, spinal disc center new jersey, artificial disc replacement, artificial disc replacement specialist, endoscopic spine surgeon, endoscopic spine surgeon new jersey, new jersey spine surgeon, best spine surgeon in new jersey, minimally invasive spine surgery, MISS New Jersey, cervical spine surgery, cervical lamino-foraminatomy, cervical radiculopathy, artificial cervical disc replacement, anterior cervical discectomy and fusion, posterior cervical discectomy and fusion, lumbar microdiscectomy, lumbar laminectomy, minimally invasive tlif, ALIF, kyphoplasty, SI joint fusion, facet joint injections, treatment for neck pain, treatment for back pain, treatment for cervical myelopathy, treatment for cervical radiculopathy, treatment for cervical stenosis, treatment for compression fractures, treatment for degenerative disc disease, treatment for herniated disc, treatment for sciatica, treatment for si joint disorders, treatment for spinal stenosis
16777
post-template-default,single,single-post,postid-16777,single-format-standard,bridge-core-3.0,wp-schema-pro-2.7.17,qode-page-transition-enabled,ajax_fade,page_not_loaded,,qode-theme-ver-28.4,qode-theme-bridge,disabled_footer_bottom,qode_advanced_footer_responsive_1000,wpb-js-composer js-comp-ver-6.7.0,vc_responsive,elementor-default,elementor-kit-16783
 

Multilineage potential of cells from the artery wall

Yin Tintut, Zeni Alfonso, Trishal Saini, Kristen Radcliff, Karol Watson, Kristina Boström, Linda L Demer: Multilineage potential of cells from the artery wall. In: Circulation, vol. 108, no. 20, pp. 2505–2510, 2003, ISSN: 1524-4539.

Abstract

BACKGROUND: In diabetes or atherosclerosis, ectopic bone, fat, cartilage, and marrow often develop in arteries. However the mechanism is unknown. We have previously identified a subpopulation of vascular cells (calcifying vascular cells, CVC), derived by dilutional cloning of bovine aortic medial cells, and showed that they undergo osteoblastic differentiation and mineralization. We now show that CVC have the potential to differentiate along other mesenchymal lineages.

METHODS AND RESULTS: To determine the multilineage potential of CVC, molecular and functional markers of multiple mesenchymal lineages were assessed. Chondrogenic potential of CVC was evidenced by expression of types II and IX collagen and cytochemical staining for Alcian blue. Leiomyogenic potential of CVC was evidenced by the expression of smooth muscle-alpha actin, calponin, caldesmon, and myosin heavy chain. Stromogenic potential of CVC was evidenced by the ability to support growth of colony-forming units of hematopoietic progenitor cells from human CD34+ umbilical cord blood cells for a period of 5 weeks. Adipogenic potential was not observed. CVC were immunopositive to antigens to CD29 and CD44 but not to CD14 or CD45, consistent with other mesenchymal stem cells. CVC retained multipotentiality despite passaging and expansion through more than 20 to 25 population triplings, indicating a capacity for self-renewal.

CONCLUSIONS: These results suggest that the artery wall contains cells that have the potential for multiple lineages similar to mesenchymal stem cells but with a unique differentiation repertoire.

Links

BibTeX (Download)

@article{pmid14581408,
title = {Multilineage potential of cells from the artery wall},
author = {Yin Tintut and Zeni Alfonso and Trishal Saini and Kristen Radcliff and Karol Watson and Kristina Boström and Linda L Demer},
doi = {10.1161/01.CIR.0000096485.64373.C5},
issn = {1524-4539},
year  = {2003},
date = {2003-11-01},
urldate = {2003-11-01},
journal = {Circulation},
volume = {108},
number = {20},
pages = {2505--2510},
abstract = {BACKGROUND: In diabetes or atherosclerosis, ectopic bone, fat, cartilage, and marrow often develop in arteries. However the mechanism is unknown. We have previously identified a subpopulation of vascular cells (calcifying vascular cells, CVC), derived by dilutional cloning of bovine aortic medial cells, and showed that they undergo osteoblastic differentiation and mineralization. We now show that CVC have the potential to differentiate along other mesenchymal lineages.

METHODS AND RESULTS: To determine the multilineage potential of CVC, molecular and functional markers of multiple mesenchymal lineages were assessed. Chondrogenic potential of CVC was evidenced by expression of types II and IX collagen and cytochemical staining for Alcian blue. Leiomyogenic potential of CVC was evidenced by the expression of smooth muscle-alpha actin, calponin, caldesmon, and myosin heavy chain. Stromogenic potential of CVC was evidenced by the ability to support growth of colony-forming units of hematopoietic progenitor cells from human CD34+ umbilical cord blood cells for a period of 5 weeks. Adipogenic potential was not observed. CVC were immunopositive to antigens to CD29 and CD44 but not to CD14 or CD45, consistent with other mesenchymal stem cells. CVC retained multipotentiality despite passaging and expansion through more than 20 to 25 population triplings, indicating a capacity for self-renewal.

CONCLUSIONS: These results suggest that the artery wall contains cells that have the potential for multiple lineages similar to mesenchymal stem cells but with a unique differentiation repertoire.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
No Comments

Sorry, the comment form is closed at this time.